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Plasmid#224537PurposeA Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pADepositorInsertIRES-(n2)StayGold(c4)-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1289-AAV-dCjCas9
Plasmid#223138PurposeAAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax-ex1
Plasmid#222141PurposeAdenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Cterm_His
Plasmid#220487Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
Plasmid#220477Purposeheterologous expression of SSAT1 under endogenous speG E. coli promoterDepositorInsertSSAT1 (SAT1 Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Nterm_His
Plasmid#220482Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PKM1
Plasmid#220920PurposeNeuronal specific expression of PKM1DepositorInsertPKM1 (PKM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PGK1
Plasmid#220919PurposeNeuronal specific expression of PGK1DepositorInsertPGK1 (PGK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV DJ-1 C106A
Plasmid#220914PurposeExpresses DJ-1 with the C106A mutationDepositorInsertDJ-1 (PARK7 Human)
UseTagsExpressionMammalianMutationC106APromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-C1-Nir1-LNS2
Plasmid#220085Purposehigh affinity PA biosensor with far-red fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsiRFP670-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail
Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-C389S-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationC389SdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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