-
Plasmid#20944Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationH134RPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPDK3860
Plasmid#199245PurposeModified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) geneDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationPromoterpPEBVAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H3
Plasmid#79514PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDSDepositorInsertLXR Alpha (NR1H3 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_81
Plasmid#60073PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS--GFP-p300
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2-BSD
Plasmid#191101PurposeLentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD markerDepositorInsertNRCH-PE2-BSD
UseCRISPR and LentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev
Plasmid#118478PurposeddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.DepositorInsertRAB-AKARev
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-miRFP670nano-miRFP670
Plasmid#216131PurposeLentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.DepositorInsertEF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TB:7xE-Box:MiniP
Plasmid#133880Purpose7 copies of the E-box motifDepositorInsert7 copies of the E-box motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorInsertLyn-RAB-EKARev
UseTagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1
Plasmid#79503PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDSDepositorInsertYAP1 (YAP1 Human)
UseMultisite gateway entry cloneTagsFLAGExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-2xFYVE_hrs
Plasmid#67676PurposeMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrsDepositorInsert2xFYVEhrs
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VP16
Plasmid#183917PurposeVP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VP16 (UL48 Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP
Plasmid#169332PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
UseTagsHaloTagExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165887PurposeG418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS-K-ras
Plasmid#11549DepositorInsertv-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
UseRetroviralTagsExpressionMutationG12DPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only