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Plasmid#91138PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2B-Venus
Plasmid#87614PurposeVenus (YFP) fused to histone H2B.DepositorInsertH2B-Venus (H2BC8 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-3xFlag-IFIT5
Plasmid#53561Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags3xFLAG, 6xHIS, and TEV siteExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ngn3ER-PuroR-mCherry
Plasmid#197385PurposeTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)DepositorInsertNgn3ER-2A-PuroR-2A-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Lysosomes-20
Plasmid#57793PurposeLocalization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLAMP1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD50E in LAMP1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSyn1-EGFP
Plasmid#153205PurposeHuman synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM68
Plasmid#133657PurposeIntegration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.DepositorInsertp1k-TGL3-mCherry (TGL3 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB-Dcp1b
Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorInsertmRNA decapping enzyme 1b (DCP1B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 TOMM22
Plasmid#157762PurposeExpression of TOMM22:mcherry in cellsDepositorInsertTOMM22 (TOMM22 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME2f-mRuby2-ST
Plasmid#170160PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME2f-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM5
Plasmid#133635PurposeIntegration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-GFP (CHS5 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Paxillin-22
Plasmid#57796PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertPaxillin
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterSP44sAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2520
Plasmid#91077PurposeModule B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterOsU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-4082NES(ForAMPK)
Plasmid#170310PurposeThe lentiviral vector for AMPK FRET-biosensorDepositorInsert4082NES (FRET biosensor for AMPK activity)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT-mGold
Plasmid#183987PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only