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Plasmid#84549PurposeHybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertT2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T4U2-YFP
Plasmid#84551PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFPDepositorInsertU2T4U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U4T2-YFP
Plasmid#84553PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFPDepositorInsertT2U4T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T2-YFP
Plasmid#84543PurposeHybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod-Doc(WT)
Plasmid#157675PurposeExpression of XMod-Doc wild type with His6x tag and SpyTag.DepositorInsertXMod-Doc Wild type with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-Coh-His-SpyTag
Plasmid#157673PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.DepositorInsertCohesin (R.f) with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY-WT-no charge
Plasmid#160539PurposeExpression of inserted PLY variantDepositorInsertPLY-WT-no charge
UseTagsHis-TagExpressionBacterialMutationK279A, Q280A, D283A, N284APromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY-WT-charge+
Plasmid#160538PurposeExpression of inserted PLY variantDepositorInsertPLY-WT-charge+
UseTagsHis-TagExpressionBacterialMutationW278K, L282KPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-N342W
Plasmid#160536PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-N342W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, N342WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-L340W
Plasmid#160535PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-L340W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, L340WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only