Addgene

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Use

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE

Plasmid

#82718

Purpose

Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe B-Raf V600E

Plasmid

#17544

Depositor

Channing Der

Article

Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

Insert

B-Raf (BRAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V600E

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-cTNT-Cre-miR122 TS

Plasmid

#209784

Purpose

Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Tags

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-EGFP

Plasmid

#14121

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Mito-CanlonicSF/pCMV-myc-mito

Plasmid

#224465

Purpose

To explore mitochondrial lactate dynamics

Depositor

Alejandro San Martín

Article

Rauseo et al bioRxiv 2024.07.19.604361

Insert

CanlonicSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

*

Promoter

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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OMM-V5-LOV-Turbo

Plasmid

#199665

Purpose

expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

AKAP1 transmembrane domain and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pIMVAu1

Plasmid

#98301

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPAICS-2XStrep

Plasmid

#125683

Purpose

expresses human PAICS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PAICS (PAICS Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

Plasmid

#172316

Purpose

CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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2xFKBP-mCh-KDEL

Plasmid

#173010

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-mCh-KDEL

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-KPTN

Plasmid

#87041

Purpose

mammalian expression of KPTN

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

KPTN (KPTN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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NgOE FUNgGW

Plasmid

#92231

Purpose

Lentiviral expression of human Ng

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Mutation

Promoter

SYN

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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