Addgene

Showing: 6181 - 6200 of 6450 results

Results per page:

LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Use

Tags

His and LOV2

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
EphA2-MT

Plasmid

#108851

Purpose

Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Use

Tags

Labeled with mTurquoise on the C-Terminus via a 1…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
EphA2-YFP

Plasmid

#108852

Purpose

Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Use

Tags

Labeled with eYFP on the C-Terminus via a 15 amin…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GNA11_G-alpha

Plasmid

#109911

Purpose

Protein expression and purification of GNA11_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA11_G-alpha (GNA11 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(R111G)

Plasmid

#110187

Purpose

Expression of mouse MeCP2 (R111G) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arg 111 Gly

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(R306C)

Plasmid

#110188

Purpose

Expression of mouse MeCP2 (R306C) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arg 306 Cys

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(deltaN)

Plasmid

#110189

Purpose

Expression of mouse MeCP2 (deltaN) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta N terminus

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(deltaNC)

Plasmid

#110190

Purpose

Expression of mouse MeCP2 (deltaNC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-PYL1-sfGFP-Coilin

Plasmid

#122177

Purpose

expression of PYL1-SfGFP-Coilin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Coilin (COIL Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Mutation

Promoter

PGK

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Use

Tags

His6-TEV

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-FLAG-MYO1D

Plasmid

#125125

Purpose

Expresses human FLAG-MYO1D

Depositor

Bruce Beutler

Article

McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

Insert

MYO1D (MYO1D Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin beta6 777T pcDNA1 neo

Plasmid

#13582

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Use

Tags

Expression

Mammalian

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin beta6 777T pBabe puro

Plasmid

#13583

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Promoter

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-nAChr-Alpha6-mCherry

Plasmid

#50480

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

mCherry fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pECE beta1 R760A

Plasmid

#50511

Depositor

Kenneth Yamada

Article

Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

Insert

integrin beta1 (ITGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

R760A

Promoter

SV40

Availability

Academic Institutions and Nonprofits only
pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Article

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Use

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RASEFIR-HA

Plasmid

#46863

Purpose

expresses mouse IL-17RA with SEFIR deletion

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-delta-SEFIR (Il17ra Mouse)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Deletion of SEFIR domain (AA 394-485)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge