Showing: 6181 - 6200 of 52850 results
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Plasmid#48227PurposedCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
UseCRISPRTags2A-neo, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pAC2-dual-dCas9VP48-sgExpression
Plasmid#48236PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promotersDepositorInsertdCas9VP48
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCool-hDDB1-BPB
Plasmid#48639PurposeExpresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)DepositorInserthDDB1-BPB (DDB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro)-Omi-mCherry
Plasmid#48685PurposeExpresses fusion protein in mitochondria inter-membrane space, and is released upon MOMP.DepositorInsertOmi (HTRA2 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1I304N
Plasmid#48692PurposeExpresses hFMRP iso 1 I304NDepositorInsertFlagHA FMR1 Iso 1 I304N (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFXR1
Plasmid#48694PurposeExpresses hFXR1DepositorInsertFlagHA FXR1 (isoform b) (FXR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N45
Plasmid#48718PurposeBacterial expression of Lacritin protein with 45 AA removed from N terminusDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationDeleted first 45 amino acids of mature lacritin w…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N71
Plasmid#48720PurposeBacterial expression of Lacritin protein with 70 AA removed from the N-terminusDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationDeleted first 71 amino acids of mature lacritin w…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-V69S
Plasmid#48721PurposeBacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationValine 69 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I73S
Plasmid#48722PurposeBacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 73 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-I98S
Plasmid#48723PurposeBacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 98 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-L108S
Plasmid#48725PurposeBacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationLeucine 108 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-F112S
Plasmid#48727PurposeBacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationPhenylalanine 12 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-I68S/I73S
Plasmid#48728PurposeBacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 68 and 73 to SerinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-L108S/L109S/F112S
Plasmid#48730PurposeBacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationLeucine 108 and 109 to Serine; phenylalanine 112 …PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhTTP
Plasmid#48738PurposeExpresses hTTPDepositorInsertTTP (ZFP36 Human)
UseTagsFLAG, HA, and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/A
Plasmid#48792PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygroDepositorInsertYFP-synhDAT-S/A (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/D
Plasmid#48793PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygroDepositorInsertYFP-synhDAT-S/D (SLC6A3 Human)
UseTagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 6181 - 6200 of 52850 results