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pCINeo-gp78 Mutant G2BR

Plasmid

#185355

Purpose

Assessing cellular requirement for G2BRgp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Use

Tags

GST

Expression

Mammalian

Mutation

aa 595-600 KRFLNK to AGAAGG

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 429-611

Plasmid

#185354

Purpose

GST fusion protein for quantification of gp78 levels

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 429-611 only

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1 A397D

Plasmid

#185342

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

A397D

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRAUP1

Plasmid

#185343

Purpose

Expression of soluble G2BR, amino acids 378-404 of human AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 378-404 (G2BR) (AUP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 378-404 only

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG DTM

Plasmid

#185337

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 24-45, corresponding to the hairpi…

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-DSP

Plasmid

#185549

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting DSP

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

DSP gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(WT)

Plasmid

#136327

Purpose

Mouse MyoGEF-WT in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12

Plasmid

#105593

Purpose

retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

silent mutations to make it resistant to the targ…

Promoter

MSCV-LTR

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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xCIAR_pcDNA5/FRT/TO

Plasmid

#86504

Purpose

Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

xCIAR (SOS1 Human)

Use

Frt

Tags

Expression

Mammalian

Mutation

F929A and T968L in SOScat

Promoter

CMV (tet operator)

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE_HRASV12_Hyg

Plasmid

#195143

Purpose

HRas G12V overexpression vector with hygroymycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

hRAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-2P

Plasmid

#179461

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pW17

Plasmid

#158207

Purpose

2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DATDCUE

Plasmid

#185340

Purpose

Assessing domain requirement sof AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 90-337 corresponding to the acyl t…

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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