Addgene

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pMA-SpCas9-g8

Plasmid

#80791

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Use

Tags

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9 iFE

Plasmid

#190138

Purpose

Expresses SpCas9-POLB (iFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9-POLB (HiFi iFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mTHC

Plasmid

#112620

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Tags

mTeal (mTFP1)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-B19M

Plasmid

#59923

Purpose

Expression vector for Rabies virus SAD B19 matrix protein gene

Depositor

Ian Wickersham

Insert

Rabies virus matrix protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCAT.015

Plasmid

#119558

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9-VRER_2A_GFP

Plasmid

#75475

Purpose

Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

Depositor

Marc Tessier-Lavigne

Article

Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

Insert

Cas9-VRER

Use

CRISPR

Tags

2A_GFP

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

CAG

Available since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

Plasmid

#188541

Purpose

Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

gRNA

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

U6

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10B-HA

Plasmid

#209216

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10B (ATP10B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-PCAF-C2

Plasmid

#134572

Purpose

Expresses YFP-PCAF fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

PCAF (Kat2b Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-C

Plasmid

#67512

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type C envelope coating virus particle with NeuRet.

Depositor

Kazuto Kobayashi

Article

Kato et al Hum Gene Ther. 2011 Dec;22(12):1511-23.

Insert

Fusion Glycoprotein type C

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Expression

Mutation

Promoter

CAGGS

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpo

Plasmid

#125576

Purpose

Expresses Flpo under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

FLPo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD104_Asn152Ser_pCMV6Entry-hIRG1

Plasmid

#124877

Purpose

Expresses hCAD Asp152Ser mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Asn152 to Ser

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAMKIIa-TetOn3G

Plasmid

#133727

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

pCamk2a

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9-NG

Plasmid

#171692

Purpose

Expresses nSpCas9-NG in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9-NG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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