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Plasmid#32827DepositorInsertAMOT p130 (AMOT Human)
UseTags2xHAExpressionMammalianMutationE5D, G858D, deletion of amino acids 410-751PromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-SREBP1
Plasmid#33357DepositorInsertSREBP1 (Srebf1 Cricetulus griseus (hamster))
UseTagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCR8-Magneto2.0
Plasmid#74333PurposeFor gateway cloning Magneto2.0 into destination vectorDepositorInsertMagneto2.0
UseGateway vectorTagsFlagExpressionMutationTRPV4: delta 760-871PromoternoneAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH4
Plasmid#52529Purposeserves as PCR-template to generate HR donors for C-terminal 2xFLAG-tagDepositorInsert2x FLAG-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AILR-LplA-ER
Plasmid#42574DepositorInsertLplA(AILR) with ER-retention sequence KDEL
UseTagsFLAGExpressionMutationCompared to wild-type LplA: tryptophan 37 to alan…PromoterCAGAvailable sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-G
Plasmid#40027DepositorInsertsGFP exon 1
GFP exon 2
beta-globin intron
UseTagsExpressionMammalianMutationinsertion of a loxP site, nucleotides 1-274, and …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV intron renilla TRICK CTE polyA
Plasmid#84443PurposeTRICK reporter mRNADepositorInsertRenill-TRICK
UseLuciferase; FrtTagsExpressionMammalianMutationPromoterTet-CMVAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-PSPC1
Plasmid#46321DepositorInsertPSPC1 (PSPC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-kanMX6
Plasmid#39293DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-myc-Rbx1
Plasmid#29506DepositorInsertRbx1 (Rbx1 Mouse)
UseTagsHis and mycExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-His3MX6
Plasmid#41604DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120391PurposepAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti GFP-AMOT p130 Y242/287A
Plasmid#32829DepositorInsertAMOT p130 (AMOT Human)
UseLentiviralTagsGFP-AMOTExpressionMammalianMutationY242/287APromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP
Plasmid#41615DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP(S65T), TRP1 reverse orientation, and yeast AD…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-T
Plasmid#36885DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIW1
Plasmid#52530Purposeserves as PCR-template to generate HR donors for C-terminal Twin-Strep tagDepositorInsertTwin-STREP-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only