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TFORF1555

Plasmid

#143825

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP110 (SP110 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1554

Plasmid

#143294

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP110 (SP110 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-Unstructured-EIF3B-465-552

Plasmid

#136029

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Delta-Hairpin

Plasmid

#136030

Purpose

EIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-1st-Hairpin

Plasmid

#136031

Purpose

EIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Disrupted-Hairpin

Plasmid

#136032

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Restored-Hairpin

Plasmid

#136033

Purpose

EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-A:U-Hairpin

Plasmid

#136034

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-465-552-Unstructured

Plasmid

#136028

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused downstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S

Plasmid

#222715

Purpose

Expression AtRBX1-T7, AtASK1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
AtASK1 (SKP1 Mustard Weed)
AtCUL1 (CUL1 Mustard Weed)

Use

Tags

S-Tag and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0641

Plasmid

#143232

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

THAP11 (THAP11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CREB3L1

Plasmid

#214767

Purpose

Mammalian expression of human CREB3L1

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

CREB3L1 (CREB3L1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-H2BmCherry-OptoJNKi

Plasmid

#89741

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Optogenetics

Tags

Histone2B and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PIK3CD gRNA (BRDN0001146545)

Plasmid

#76165

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CD

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CD (PIK3CD Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CD gRNA (BRDN0001146527)

Plasmid

#76164

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CD

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CD (PIK3CD Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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