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pCalpha EV (PKA catalytic subunit Calpha)
Plasmid#15310DepositorInsertPRKACA (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
PKCalpha.FLAG
Plasmid#10805DepositorInsertPKC alpha (PRKCA Bovine)
UseTagsFLAGExpressionMammalianMutationD332G-please see comments belowPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.PKD3.RNAi
Plasmid#10818DepositorInsertPKD3 RNAi (PRKD3 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.S738A/S742A
Plasmid#10814DepositorInsertPKD (PRKD1 Human)
UseTagsHAExpressionMammalianMutationActivation loop inactive: S738A and S742A; L520PPromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCbeta EV (PKA catalytic subunit Cbeta)
Plasmid#15311DepositorInsertPRKACB (Prkacb Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
cavin-3-HA
Plasmid#27711DepositorInsertcavin-3 (CAVIN3 Human)
UseTagsHAExpressionMammalianMutationR8PPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.deltaPH
Plasmid#10811DepositorInsertPKD (PRKD1 Human)
UseTagsHAExpressionMammalianMutationPH domain deleted (amino acids 417-545); F94I, V4…PromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
PKCalpha.K/N.FLAG
Plasmid#10806DepositorInsertPKC alpha (PRKCA Bovine)
UseTagsFLAGExpressionMammalianMutationKinase dead mutant: K368N (Lys 368 to Asn)PromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PACT
Plasmid#50352Purposebacterial expression of PACTDepositorInsertPACT (PRKRA Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorInsertPRKN (PRKN Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
enz.099-PKA
Plasmid#154048PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsHis, MBPExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCb1-mEGFP-N1
Plasmid#112265PurposeITRACKbeta/IDOCKSbeta Donor constructDepositorInsertprotein kinase c beta (Prkcb Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MARCKS(1-40)-paGFP
Plasmid#18695DepositorInsertMARCKS(1-40) (MARCKS Human)
UseTagsphotoactivatable GFPExpressionMammalianMutationA206K mutation in paGFPPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.S738E/S742E
Plasmid#10810DepositorInsertPKD (PRKD1 Human)
UseTagsHAExpressionMammalianMutationActivation loop active: S738E and S742E (bp 2212-…PromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.K/W
Plasmid#10809DepositorInsertPKD (PRKD1 Human)
UseTagsHA and Myc (inserted between amino acids 33&3…ExpressionMammalianMutationKinase dead mutant: K612W (Lys 612 to Trp) (bp183…PromoterCMVAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCg-C1
Plasmid#112266PurposeITRACKgamma Donor ConstructDepositorInsertprotein kinase c gamma (Prkcg Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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XE107 XPKC alpha-GFP-CS2+
Plasmid#16743DepositorInsertPKC-alpha (prkca Frog)
UseXenopus expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENT L1-Kozak-ERK-KTR-L2
Plasmid#186353PurposeEntry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertERK Kinase Translocation Reporter (MAPK1 Synthetic, Human)
UseProtein expressionTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterpCinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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Cas9-GFP_sg_mAMPKa1
Plasmid#79004PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.DepositorInsertPrkaa1 (Prkaa1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9-GFP_sg_mAMPKa2
Plasmid#79005PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.DepositorInsertPrkaa2 (Prkaa2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCa-mEGFP-N1
Plasmid#112269PurposeIDOCKSalpha Donor ConstructDepositorInsertProtein Kinase C alpha (PRKCA Bovine)
UseTagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AMPK-β1-FLAG
Plasmid#40602DepositorInsertAMPK-β1 (Prkab1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1
Plasmid#14538DepositorInsertMKK7a1 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC lambda (JCE590)
Plasmid#89775Purposeyeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC lambda (Prkci Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7b1
Plasmid#14622DepositorInsertMKK7b1 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only
