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pJH2069

Plasmid

#179224

Purpose

Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK138

Plasmid

#173746

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1850

Plasmid

#154328

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1849

Plasmid

#154327

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

Plasmid

#159898

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJA54

Plasmid

#60212

Purpose

cleavage near e1350 locus in sqt-1

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Benjamin Schuster, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSM12

Plasmid

#139035

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

dendra2 (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16b

Plasmid

#31504

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16b specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGC76

Plasmid

#19641

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<4xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159873

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2215 - Intron GG3 - 900 bp

Plasmid

#159882

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - 900 bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pXF130

Plasmid

#139041

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGC31

Plasmid

#19678

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)-L4440

Use

RNAi; Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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