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Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianMutationPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CD81-10
Plasmid#55012PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorInsertCD81 (CD81 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP53
Plasmid#22606DepositorInsertUSP53 (USP53 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationnot FL, missing first 65 aaPromoterCMV/TOAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SC35-cMyc
Plasmid#44721DepositorInsertSC35 (SRSF2 Human)
UseTagshis and mycExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET302-6His-dCas9-Halo
Plasmid#72269PurposeEncodes a Halo-labeled fusion of nuclease deficient Cas9DepositorInsertHis6-dCas9-Halo
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs T3950D
Plasmid#83320PurposeDNA-PKcs T3950ADepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutationActivation loop phosphorylation site 3950 substit…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6c
Plasmid#207853PurposeMammalian expression of PE6c prime editorDepositorInsertPE6c
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRE-luciferase
Plasmid#26731PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.DepositorInsertHypoxia Responsive Elements (3)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
UseTagsExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-hYAP1
Plasmid#17843DepositorInsertYAP (YAP1 Human)
UseTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pMXs-puro GFP-p62
Plasmid#38277DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
UseTags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas ServiceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-RC3AI
Plasmid#78908PurposeIndicator of caspase-3-like protease activityDepositorInsertRC3AI
UseLentiviralTagsHAExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only