Addgene

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Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Use

Tags

GST and thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCDM8-GlyCAM-1/IgG

Plasmid

#17841

Depositor

Steven Rosen

Article

Bistrup et al J Cell Biol. 1999 May 17. 145(4):899-910

Insert

GlyCAM-1/IgG (Glycam1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2009

Availability

Academic Institutions and Nonprofits only
GST-PRMT7 LDIG69AAAA

Plasmid

#61560

Purpose

Hs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2014 Nov 21;289(47):32604-1

Insert

PRMT7 (PRMT7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LDIG 69-72 AAAA

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Flag-M4-AR-K313A-K318A

Plasmid

#171260

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Lys313Ala-Lys318Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 CLV3

Plasmid

#115485

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_2

Plasmid

#36350

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pDONR_P4-P1R_5'arm_apobb.1

Plasmid

#128662

Purpose

apobb.1 homology arm 5' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

Genomic sequence upstream of apobb.1 stop codon (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 G260E mutant

Plasmid

#67610

Purpose

bacterial expression of GST-PRMT9 G260E Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

G260E double E loop mutant

Promoter

tac

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 D258G mutant

Plasmid

#67609

Purpose

bacterial expression of GST-PRMT9 D258G Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D258G double E loop mutant

Promoter

tac

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
5aa SunTag VP64 AP3

Plasmid

#115484

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_3

Plasmid

#36351

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
β2AR-PPI-CALPon1.0 (APEX)

Plasmid

#213406

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEG302-JMJ14-ZF

Plasmid

#200913

Purpose

Express JMJ14-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

JMJ14

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-HD2A-ZF

Plasmid

#200915

Purpose

Express HD2A-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

HD2A

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version1

Plasmid

#197937

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Stx3

Plasmid

#186685

Purpose

Expresses Syntaxin 3 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Syntaxin 3

Use

Tags

6 his tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET15 PemrR-gfp biosensor

Plasmid

#185793

Purpose

The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

Depositor

Steven Hallam

Article

Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

Insert

pEmrR-gfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

PemrR

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-K630A-K632A

Plasmid

#171263

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRMT9 deltaTPR mutant

Plasmid

#67614

Purpose

bacterial expression of GST-PRMT9 missing residues 21-139

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

truncation mutant missing aa 21-139

Promoter

tac

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 deltaN (aa 21-350)

Plasmid

#67611

Purpose

bacterial expression of GST-PRMT9 missing residues 21-350

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

truncation mutant missing aa 21-350

Promoter

tac

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only