-
Plasmid#127852PurposeBacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVSp1
UseTags3C Cleavage site, His Tag, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES EGFP
Plasmid#130707PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES EGFP
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#90964Purposeyeast vectorDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationPromoternativeAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-3'ss mut
Plasmid#67644PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation3' splice site muattionPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa38_1322
Plasmid#49698DepositorInsertECF38_1322
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(3'')-Ia
Plasmid#112879PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRa27_4265
Plasmid#49682DepositorInsertECF27_4265
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOT2 3GLA
Plasmid#71764Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistantDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir129-2-wt
Plasmid#46730DepositorInsertmir129-2-wt (MIR129-2 Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGex4t2 with full-length SUUR
Plasmid#112990PurposeExpresses fusion of GST and full-length Drosophila SUURDepositorInsertSuUR (SuUR Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1016
Plasmid#92255PurposePlasmid for expression of HA-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsHAExpressionMammalianMutation.C-terminal effector domain (CED): aa 1369-1690PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 C140S (#622)
Plasmid#99947Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationC140SPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-RFP-miR-223 sponge
Plasmid#97150PurposeFor miR-223 knock downDepositorInsertmiR-223 sponge
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ERBB4 (E563K)
Plasmid#29541DepositorInsertERBB4 (ERBB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCh-133
Plasmid#92256PurposePlasmid for expression of NHA-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutation.C-terminal effector domain (CED): aa 1369-1690PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBS931 TDP43 RRM-GFP F-S
Plasmid#107799PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll phenylalanine to serinePromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYG026
Plasmid#65328Purposereporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.DepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Plxnb3-ISH-2kb
Plasmid#68037PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertPlexin-B3 (Plxnb3 Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only