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TRS6E1b-luc(-732/-721) mutant 3

Plasmid

#45386

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-723/-721 mutated from tgt to aca

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 2

Plasmid

#45385

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated at -730/-728 from aca to tgt

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant1

Plasmid

#45384

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI1 promoter -732/-721 mutated at -732/-731

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated -732/-731 from ag to tc

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
GMR-hopscotch

Plasmid

#42062

Depositor

Norbert Perrimon

Article

Bach et al Genetics. 2003 Nov;165(3):1149-66.

Insert

hopscotch (hop Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL* EPSM

Plasmid

#27005

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203; T179V

Promoter

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL* WT

Plasmid

#27042

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-203

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag Smad3 AAGY

Plasmid

#27027

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL AAGY

Plasmid

#27041

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM

Plasmid

#27222

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A

Promoter

Available since

Dec. 21, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 AAGY

Plasmid

#27013

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

P181A P182A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
HsCLYBL(aa23-340)-His

Plasmid

#111288

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RALA G23D

Plasmid

#122926

Purpose

Bacterial expression of RALA G23D

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

G23D

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL1765 (pSPIN-R, R*MmeI)

Plasmid

#160734

Purpose

Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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