Addgene

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-CALM2

Plasmid

#23734

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM2 (CALM2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FGFR1

Plasmid

#23922

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR1 (FGFR1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W211G and S351F compared to insert sequence provi…

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAGI1

Plasmid

#23523

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FLT1

Plasmid

#23912

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S755P

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-BUB1B

Plasmid

#23858

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DDR1

Plasmid

#23910

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR1 (DDR1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK1

Plasmid

#23498

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

missing first codon

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT1

Plasmid

#23752

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS-PDGFRb559-562del-IRES2-EGFP

Plasmid

#204355

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion).

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

Tags

Expression

Mutation

559-562 deletion

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-PDGFRb-HA

Plasmid

#204350

Purpose

Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-PDGFRb559-562del-HA

Plasmid

#204351

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

AAV

Tags

HA

Expression

Mutation

559-562 deletion

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZAK

Plasmid

#23709

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAK (MAP3K20 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKR

Plasmid

#23452

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKR (EIF2AK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

aa494-511 missing

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DLG3

Plasmid

#23451

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DLG3 (DLG3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G381A,G629T

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT3

Plasmid

#23539

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S2R

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K8

Plasmid

#23538

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

1MM

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
SHP2(T253M/Q257L)@pLKO-Trex-SBP

Plasmid

#85458

Purpose

SHP2 mutant gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

T253M/Q257L

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Native promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge