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Plasmid#187126PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationY355G/H356G, delta3291-3298PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta3291-3298
Plasmid#187124PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationdelta3291-3298PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG
Plasmid#187122PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationY355G/H356GPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0760
Plasmid#185629PurposeModified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamianaDepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0751
Plasmid#185628PurposeModified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaIDepositorInsertlacZ
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0001
Plasmid#185625PurposeMoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoterDepositorInsertSpCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2G2A
Plasmid#174334Purposeencodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationmutation of a.a 2 Gly to AlaPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FG11F-C-FLAG-hNKD2
Plasmid#174330Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseLentiviralTagsFLAG TagExpressionMammalianMutationPromoterUbCAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only