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pCMV-Neo-Bam p53 R273H

Plasmid

#16439

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R273H (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R273H

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mCasp8

Plasmid

#208782

Purpose

Inducibly expresses mouse caspase8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase8 Cry2 (Casp8 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mMLKL

Plasmid

#208784

Purpose

Inducibly expresses mouse MLKL fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mMLKL Cry2 (Mlkl Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-SEP-mRuby3

Plasmid

#200939

Purpose

endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

LAMP1 (LAMP1 Human)
SEP-mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.2 myc

Plasmid

#12577

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-opto-mRipK3

Plasmid

#208783

Purpose

Inducibly expresses mouse RipK3 fused to light activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mRipK3 Cry2 (Ripk3 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.5 myc

Plasmid

#12579

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.5 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.4 myc

Plasmid

#12578

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Insert

mSin1.4 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
PGK-H2BmCherry

Plasmid

#21217

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-mCherry

Expression

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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Brm WT-Flag pShuttle

Plasmid

#122105

Purpose

Expression of full length Brm in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

BRM (SMARCA2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti4-Puro-H19

Plasmid

#200835

Purpose

Lentiviral vector expressing human long non-coding RNA H19

Depositor

Reinier Boon

Article

Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

Insert

Human long non-coding RNA H19

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Use

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

Plasmid

#46594

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-17 Decoy (MIR17, Mir17 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2_mSTING_scrambled_gRNA_1

Plasmid

#196627

Purpose

Scrambled control 1 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-mito)

Plasmid

#214423

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito

Use

Lentiviral

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12.D11

Plasmid

#221151

Purpose

cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Integrin beta 2 - mYFP

Plasmid

#8638

Depositor

Timothy Springer

Article

Kim et al Science 2003 Sep 19;301(5640):1720-5.

Insert

Integrin beta 2 (ITGB2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

stop codon of beta2 was replaced with AgeI restri…

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R248W

Promoter

Available since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge