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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4900
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIntegrin beta 2
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Alt nameCD18
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SpeciesH. sapiens (human)
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Insert Size (bp)3500
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Mutationstop codon of beta2 was replaced with AgeI restriction site. Leu221 of YFP was replaced with lysine by PCR to generate monomeric YFP.
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Entrez GeneITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
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Tag
/ Fusion Protein
- YFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Integrin beta 2 - mYFP was a gift from Timothy Springer (Addgene plasmid # 8638 ; http://n2t.net/addgene:8638 ; RRID:Addgene_8638) -
For your References section:
Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982