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Addgene

Integrin beta 2 - mYFP
(Plasmid #8638)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8638 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEYFP N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrin beta 2
  • Alt name
    CD18
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Mutation
    stop codon of beta2 was replaced with AgeI restriction site. Leu221 of YFP was replaced with lysine by PCR to generate monomeric YFP.
  • Entrez Gene
    ITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin beta 2 - mYFP was a gift from Timothy Springer (Addgene plasmid # 8638 ; http://n2t.net/addgene:8638 ; RRID:Addgene_8638)
  • For your References section:

    Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982