-
Plasmid#216848PurposeThis plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diffDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterOct4Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD40L-SrtA
Plasmid#121167PurposepMP71 vector expressing Tomato - P2A - CD40L - SrtA - flagDepositorInsertCD40L-SrtA
UseRetroviralTagsTomato and flagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCGN N-Ras wt
Plasmid#14723DepositorInsertN-ras (NRAS Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-HaloTag7-mGFP
Plasmid#184903PurposeStable expression of HaloTag7-mGFP in mammalian cells to measure autophagic fluxDepositorInsertmonomeric EGFP
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA030
Plasmid#216010PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lck-mScarlet-I
Plasmid#98821PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorArticleInsertLck
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos2.0
Plasmid#73228PurposeGenome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052DepositorInsertsCas9 nickase
sgRNA to xylR
UseE.coli - clostridium shuttle vectorTagsExpressionMutationD10APromoterPj23119 and PthlAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-plvxt
Plasmid#119281PurposeDoxycycline-inducible human BRCA1DepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCas9
Plasmid#41815PurposeExpresses human codon optimized Cas9 nuclease for genome engineeringDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-SMARCA4
Plasmid#65391PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorInsertIRF1 (IRF1 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pUC18-mini-Tn7T-Gm-lux
Plasmid#64963Purposemini-Tn7 luxCDABE transcriptional fusion vectorDepositorInsertlux operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXIN GFP
Plasmid#132935PurposeMLV-based transfer vector expressing GFP and neomycin resistance proteinDepositorInsertGFP
UseLentiviral, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA
Plasmid#53272PurposeContains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertcrtE, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RFP-TEVprotease
Plasmid#124947PurposeExpression of RFP-TEVproteaseDepositorInsertRFP-TEVprotease
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPI
Plasmid#12254Purpose2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsEMCV IRES-EGFP cassetteExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only