Skip to main content
Addgene
Showing: 6181 - 6200 of 152043 results

  6. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Todd Klaenhammer, Rodolphe Barrangou
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

    Enlarge

  7. HOP-flash

    Plasmid
    #83467
    Purpose
    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83467-plasmid-map-sequence-id-350804

    Enlarge

  11. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

    Enlarge

  13. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

    Enlarge

  14. pKM461

    Plasmid
    #108320
    Purpose
    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108320-plasmid-map-sequence-id-210431

    Enlarge

  15. pAAV-GFAP-hChR2(H134R)-EYFP

    Plasmid
    #27054
    Purpose
    AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    GFAP
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27054-plasmid-map-sequence-id-12795

    Enlarge

  20. pHIV-dTomato

    Plasmid
    #21374
    Purpose
    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21374-plasmid-map-sequence-id-292244

    Enlarge

Showing: 6181 - 6200 of 152043 results