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Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only
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pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LHP2691 - pET30-Ub-K63A
Plasmid#32179DepositorUseTagsHISExpressionBacterialMutationK63APromoterT7Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP2690 - pET30-Ub-K48A
Plasmid#32178DepositorUseTagsHISExpressionBacterialMutationK48APromoterT7Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJJB1340
Plasmid#218593PurposeExpress a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11DepositorInsertPex22
UseTagsExpressionYeastMutationPex22(1-36)PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1357
Plasmid#218595PurposeExpress Pex11 and the degron assay: Degradation tag-YFP-ePTS1DepositorInsertPex11
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1461
Plasmid#218587PurposeExpress peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPPDepositorInsertErg12
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Kar2-moxGFP2-HDEL
Plasmid#218970PurposeGene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2DepositorInsertKAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
UseGene replacement vector for yeastTagsmoxGFP2ExpressionMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Adan
Plasmid#181733PurposeGalactose inducible expression of Prepro-3x-AdanDepositorInsertPrepro-3x-Adan
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Kar2-Beta-Amyloid
Plasmid#181707PurposeGalactose inducible expression of Kar2-Beta-AmyloidDepositorInsertKar2-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-RNQ1
Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-YES1-6stop
Plasmid#203476PurposeExpresses human YES1 kinase from a GAL10 promoter with a URA3 markerDepositorInsertYES1 (YES1 Human)
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-Bat-SL-CoVZC45_PLP
Plasmid#201939PurposeExpresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 markerDepositorInsertBat-SL-CoVZC45 PLP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCV_NS3-GS-NS4A
Plasmid#203504PurposeExpresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertHCV NS3-GS-NS4A
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HRV-B14_3C
Plasmid#203466PurposeExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHRV-B14 3C protease (HRVBgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-DUGV_OTU
Plasmid#203463PurposeExpresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 markerDepositorInsertDUGV OTU domain of L protein (DUGVsLgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCoV-NL63_PLP2
Plasmid#203502PurposeExpresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 markerDepositorInsertHCoV-NL63 PLP2 (ORF1ab )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP1
Plasmid#166103PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.DepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only