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Plasmid#211661PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgOR2B6-3_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211657PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgOR2B6-3 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-32_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211676PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-32 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-1_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211658PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TBPL1 KO 2
Plasmid#200211PurposegRNA for TBPL1 KODepositorInsertTBP1 KO 2 (Tbpl1 Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A_1208-1709_L
Plasmid#146844PurposeInsect Expression of HsTNRC6A_1208-1709DepositorInsertHsTNRC6A_1208-1709 (TNRC6A Human)
UseTagsExpressionInsectMutationone non silent mutation K1578E compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A_1208-1709-F1359A_L
Plasmid#146845PurposeInsect Expression of HsTNRC6A_1208-1709-F1359ADepositorInsertHsTNRC6A_1208-1709-F1359A (TNRC6A Human)
UseTagsExpressionInsectMutationone non silent mutation K1578E compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A-F1359A_L
Plasmid#146846PurposeInsect Expression of HsTNRC6A-F1359ADepositorInsertHsTNRC6A-F1359A (TNRC6A Human)
UseTagsExpressionInsectMutationone non silent mutation K1578E compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1-1208-siRNAres_K
Plasmid#146741PurposeMammalian Expression of HsTNRC6A_1-1208-siRNAresDepositorInsertHsTNRC6A_1-1208-siRNAres (TNRC6A Human)
UseTagsExpressionMammalianMutation254-1962 truncated version of the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-F1359A-siRNAres_K
Plasmid#146744PurposeMammalian Expression of HsTNRC6A-F1359A-siRNAresDepositorInsertHsTNRC6A-F1359A-siRNAres (TNRC6A Human)
UseTagsExpressionMammalianMutationone non silent mutation K1578E compared to the se…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkB2 guide 1
Plasmid#193593PurposeNFkB2 knockoutDepositorInsertsgNFkB2 guide 1 (NFKB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Tesmin-BioID2-3xFLAG
Plasmid#186816PurposeMouse Clgn promoter-driven expression of TESMIN-BioID2-3xFLAGDepositorInsertTesmin (Tesmin Mouse)
UseTagsBioID2-3xFLAGExpressionMammalianMutationPromotermouse Clgn promoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Tesmin-TurboID-3xFLAG
Plasmid#186817PurposeMouse Clgn promoter-driven expression of TESMIN-TurboID-3xFLAGDepositorInsertTesmin (Tesmin Mouse)
UseTagsTurboID-3xFLAGExpressionMammalianMutationPromotermouse Clgn promoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-4x(tRNA::axed{sgRNA})
Plasmid#187883PurposeGal4/UAS sgRNA expression targeting axedDepositorInsert4 sgRNAs targeting axed
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.108
Plasmid#184977PurposeExpress -Eco1 LYP1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
UseTagsExpressionYeastMutationLYP1 donor E27stopPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.109
Plasmid#184978PurposeTest effect of extending a1/a2 on LYP1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationLYP1 donor E27stop, a1/a2 length extended to 27 bpPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#189944PurposeLentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#189952PurposeLentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1373_mU6_sgRNA targeting e1
Plasmid#190684PurposesgRNA targeting enhancer 1 of MYCDepositorInsertsgRNA targeting enhancer 1 of MYC
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only