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Showing: 6081 - 6100 of 9011 results

  1. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  2. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166109-plasmid-map-sequence-id-330317

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  6. pREP81xV5_ccdB

    Plasmid
    #79019
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79019-plasmid-map-sequence-id-151964

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  7. pREP41xV5_ccdB

    Plasmid
    #79017
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79017-plasmid-map-sequence-id-151963

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  8. pREP82xV5_ccdB

    Plasmid
    #79020
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79020-plasmid-map-sequence-id-147777

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  9. pREP42xV5_ccdB

    Plasmid
    #79018
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79018-plasmid-map-sequence-id-147775

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  10. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

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  13. pAG426-GAL-TAF15

    Plasmid
    #181713
    Purpose
    Galactose inducible expression of TAF15
    Depositor
    Alejandro Chavez
    Insert
    TAF15 (TAF15 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    181713-plasmid-map-sequence-id-436302

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  14. pRS303-PADH1-AFB2

    Plasmid
    #99530
    Purpose
    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99530-plasmid-map-sequence-id-190861

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  15. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  16. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Bogumil Karas
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203882-plasmid-map-sequence-id-403835

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  17. STK25

    Plasmid
    #39075
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK25 (STK25 Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. YIp204-PADH1-AFB2

    Plasmid
    #99531
    Purpose
    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99531-plasmid-map-sequence-id-190867

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  19. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

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  20. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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Showing: 6081 - 6100 of 9011 results