Addgene

We narrowed to 24,207 results for: sta

Showing: 6081 - 6100 of 24207 results

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Blast mouse E-cadherin

Plasmid

#18804

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin (Cdh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Use

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBbB9a-GFP

Plasmid

#126112

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23113

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(S75N)

Plasmid

#99719

Purpose

Mammalian expression of HA-RagC(S75N)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

HA

Expression

Mutation

S75N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 NAPA-N SV40

Plasmid

#92282

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin1

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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ChrII_ttTi5605_loxP_myo2_neoR

Plasmid

#194056

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ttTi5605

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUA66 PcspA GFP No Linker

Plasmid

#105605

Purpose

Expresses GFP under temperature sensitive promoter PcspA

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcspA

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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ChrV_oxTi365_loxP_myo2_neoR

Plasmid

#194057

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

oxTi365

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBbB12a-GFP

Plasmid

#126115

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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SauriBE4max

Plasmid

#135969

Purpose

Expresses AncBE4max and nSauriCas9

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1 eforRed

Plasmid

#106320

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB14a-GFP

Plasmid

#126117

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB11a-GFP

Plasmid

#126114

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23150

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pZY101

Plasmid

#73932

Purpose

This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Insert

bar

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB19a-GFP

Plasmid

#126131

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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