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Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-RandE3
Plasmid#79870PurposeExpresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.DepositorInsertGFP-RandE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4/6(1)
Plasmid#73554Purposevector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)DepositorInsertshRNA against CDK4 + shRNA against CDK6
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-Lyn-SponGee
Plasmid#134776Purposemammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertLyn-SponGee
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK4mut(K35M)-3XFlag-pBabe
Plasmid#73559Purposevector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tagsDepositorInsertCDK4mut(K35M)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1
Plasmid#14538DepositorInsertMKK7a1 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_ABE_D3_empty
Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo
Plasmid#179439PurposeExpresses rTetR-KRAB(human) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-KRAB
UseTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag GCN5 Y621A/P622A
Plasmid#14425DepositorInsertGCN5 Y621A/P622A (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationY621A, P622A. Catalytically inactive acetyltransf…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
p1.1-eGFP
Plasmid#162738PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SL016: AAV-CAG-FLEX-CheRiff-GFP
Plasmid#124776PurposeFor AAV based expression of CheRiff in Cre-Positive cellsDepositorInsertCheRiff-GFP
UseAAV and Cre/LoxTagsGFPExpressionMutationPromoterCAGAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-st1CAS9-pA
Plasmid#131542PurposeExpresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized st1Cas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_BFP
Plasmid#45297DepositorInsertBFP
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS
Plasmid#79067PurposeExpress owl monkey TRIMCyp in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD8 Flag
Plasmid#74900PurposeExpress COMMD8 in mammalian cellsDepositorInsertCOMMD8 (COMMD8 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorInsertRARalpha (RARA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNEXdelta-EGFP-ApAF
Plasmid#13335DepositorInsertAplysia activating factor
UseTagsEGFPExpressionMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only