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Plasmid#183075PurposePlasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertLbCas12a guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP64-H2B-GFP
Plasmid#135605PurposeExpression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratusDepositorInsertH2B-GFP
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP-KANL
Plasmid#170164PurposeMammalian Expression of Ef1a-EGFP-KANLDepositorInsertEGFP-KANL
UseTagsExpressionMammalianMutationKANLPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-639.sensor.PGK.dNGFR.WPRE
Plasmid#85923PurposeSensor PlasmidDepositorInsertmiR-639 Sensor (MIR639 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
NSm-GFP pZK538
Plasmid#118012Purposeexpresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).DepositorArticleInsertsNSm
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Rx3(A8)-mEGFP
Plasmid#35532DepositorInsertRx3A8mEGFP
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
flGalT-SBP-EGFP_puromycin
Plasmid#65275PurposeGalT reporter only ( no hook) (RUSH system)DepositorInsertgalactosidase
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGH314_wtGFP S65T, Q80H
Plasmid#85407Purposelentiviral expression vector for wtGFP S65T-Q80HDepositorInsertwtGFP S65T, Q80H
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot10
Plasmid#79254PurposeExpresses EGFP-tagged DmNot10 in S2 cellsDepositorInsertDmNot10 (Not10 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot11
Plasmid#79253PurposeExpresses EGFP-tagged DmNot11 in S2 cellsDepositorInsertDmNot11 (Not11 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
iLID-eGFP-2xC2
Plasmid#114416Purposeanchor iLID domain to plasma membrane with slower diffusionDepositorInsertiLID domain
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMuro
Plasmid#82504PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInserteGFP-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRET.IL.IRES-EGFP (No HSV-TK)
Plasmid#1836DepositorTypeEmpty backboneUseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPROBE'-gfp[AAV]
Plasmid#40169DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only