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Showing: 6061 - 6080 of 9011 results

  2. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid
    #203506
    Purpose
    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    KUNV NS2B-GS-NS3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203506-plasmid-map-sequence-id-402629

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  3. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  4. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  5. pREP4xV5_ccdB

    Plasmid
    #79016
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79016-plasmid-map-sequence-id-147773

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  6. pREP3xV5_ccdB

    Plasmid
    #79015
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79015-plasmid-map-sequence-id-151962

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  7. pJJB1462

    Plasmid
    #218623
    Purpose
    Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease
    Depositor
    John Dueber
    Insert
    YFP
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218623-plasmid-map-sequence-id-432996

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  8. pJJB1463

    Plasmid
    #218624
    Purpose
    Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease
    Depositor
    John Dueber
    Insert
    YFP
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218624-plasmid-map-sequence-id-432999

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  9. pJJB1376

    Plasmid
    #218618
    Purpose
    Express Pex5, Pex3, Pex19, and Pex8
    Depositor
    John Dueber
    Insert
    Pex5
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218618-plasmid-map-sequence-id-431891

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  10. pJJB1577

    Plasmid
    #218619
    Purpose
    Express Pex5, Pex2, Pex17, and Pex10
    Depositor
    John Dueber
    Insert
    Pex5
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218619-plasmid-map-sequence-id-431875

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  11. pJJB1338

    Plasmid
    #218592
    Purpose
    Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22
    Depositor
    John Dueber
    Insert
    Pex22
    Use
    Tags
    Expression
    Yeast
    Mutation
    Pex22(1-36)
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218592-plasmid-map-sequence-id-432601

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  12. pJJB207

    Plasmid
    #218596
    Purpose
    Express degron assay: Degradation tag-YFP-ePTS1
    Depositor
    John Dueber
    Insert
    YFP
    Use
    Tags
    UbiY degradation tag and ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218596-plasmid-map-sequence-id-431863

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  13. pUSE-URA-Sec7-mGold

    Plasmid
    #218967
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold
    Depositor
    Benjamin Glick
    Insert
    SEC7-mGold (SEC7 Budding Yeast)
    Use
    Gene replacement vector for yeast
    Tags
    mGold
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218967-plasmid-map-sequence-id-446219

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  14. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Benjamin Glick
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Use
    Tags
    msYFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218969-plasmid-map-sequence-id-445656

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  15. pHflu1

    Plasmid
    #203878
    Purpose
    pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer
    Depositor
    Bogumil Karas
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203878-plasmid-map-sequence-id-408184

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  16. pAG426GAL-CV-B3_3C

    Plasmid
    #203499
    Purpose
    Expresses CV-B3 3C protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    CV-B3 3C protease (D1P32_gp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203499-plasmid-map-sequence-id-402652

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  17. pAG416GAL10-SV-A_3C

    Plasmid
    #203474
    Purpose
    Expresses SV-A 3C protease from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    SV-A 3C protease (Hk1_gp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203474-plasmid-map-sequence-id-402644

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  18. pAG416GAL10-HPeV_3C

    Plasmid
    #203465
    Purpose
    Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    HPeV 3C protease (polyprotein gene )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203465-plasmid-map-sequence-id-402619

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  19. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

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  20. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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Showing: 6061 - 6080 of 9011 results