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Plasmid#71752PurposeAurein1.2+36GFP-BirA fusion for E.Coli expressionDepositorInsertAurein1.2+36GFP-BirA
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 4SE
Plasmid#16230DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutation4SE: S108E, S112E, S116E, S120EPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha218-1374
Plasmid#62523PurposeN-terminal aa1-217 of Drosha is deletedDepositorInserthuman Drosha with aa 1-217 deleted (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-217PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Cacnb1 KI
Plasmid#139660PurposeEndogenous tagging of CaVβ1: N-terminal (amino acid position: S8)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cacnb2-GFP KI
Plasmid#139661PurposeEndogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE C9orf4-GFP KI
Plasmid#131472PurposeEndogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and GFP donor (Frrs1l Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheP1Egfp
Plasmid#90165Purposecontains She specific gene promoter driving expression of eGFPDepositorInsertsheP1Egfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-GFP
Plasmid#124667PurposeExpresses UBE2QL1 with C-terminal GFP tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoT-EGFP
Plasmid#159792PurposeS. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoG-EGFP
Plasmid#159791PurposeS. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SMAC-HA-eGFP
Plasmid#67488PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-SMAC-HA-eGFP
Plasmid#67491PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-MosIR
Plasmid#120516PurposedsRNA expressionDepositorInsertMos (Mos Mouse)
UseRNAiTagsExpressionMutationL170P in second MosIR repeat (see comments below)PromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFP human RASSF6b
Plasmid#37021DepositorInsertRASSF6b (RASSF6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB-EGFP
Plasmid#14199DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
M71::GFP LTNL TV
Plasmid#15618DepositorInsertM71::GFP LTNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-mGFP
Plasmid#62636Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mGFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A an…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only