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Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA, araBAD and LacO3O1 promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD_W35A-IFP2.0C
Plasmid#215755PurposeExpresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD_W35A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL_WobbleMut
Plasmid#209053PurposeGateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL
Plasmid#209047PurposeGateway-compatible entry vector, with insert of the full length TAF5 geneDepositorInsertTAF5 full length
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-∆ex8
Plasmid#209048PurposeGateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM 1996
Plasmid#217825PurposeExpresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.DepositorInsertSA-phr1
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E9
Plasmid#213472PurposeGateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editingDepositorInsertTadDE-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tadde-32aa linker-zspc…TagsNOExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FAS-GCaMP6f-WPRE
Plasmid#216277PurposeAAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only