Showing: 6021 - 6040 of 16015 results
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Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-F2A-T2A-Venus
Plasmid#62621PurposeAdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tagDepositorInsertMCS with F2A and T2A
UseAdenoviralTagsVenusExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/mKO2
Plasmid#110353PurposeDoxycycline inducible mammalian retroviral expression of mKO2 with FLAG tagDepositorInsertmonomeric Kusabira Orange 2
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneUseTags6xhisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX1
Plasmid#104975PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1745 (MXAN_1745 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX2
Plasmid#104976PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1744 (MXAN_1744 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxEH
Plasmid#104977PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1644 (MXAN_1644 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-NUP37 neo
Plasmid#220361PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37DepositorInsertNUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LEMD3_BirA_Cterm
Plasmid#221517PurposeFlag-tagged biotinylation enzyme for co-expression of Lemd3DepositorInsertLEMD3 (LEMD3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LEMD3_BirA_Nterm
Plasmid#221518PurposeFlag-tagged biotinylation enzyme for co-expression of Lemd3DepositorInsertLEMD3 (LEMD3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Gal4-HA-HIF1α(530-652)
Plasmid#24887DepositorInserthypoxia inducible factor1alpha (HIF1A Human)
UseTagsGal4dbd and HA-tagExpressionMammalianMutationpartial HIF1alpha cDNA (encoding aa 530-652)fused…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
Plasmid#72611PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37DepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33, threonine34 and serine37 to ala…PromoterAvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd1-NLS-3XFLAG-V5
Plasmid#196092PurposeExpresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 96-285 (predicted disordered …PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd3-NLS-3XFLAG-V5
Plasmid#196094PurposeExpresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 638-925 (predicted disordered…PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-DD3-NLS-3XFLAG-V5
Plasmid#196095PurposeExpresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationContains only AA 619-925 (NLS and predicted disor…PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-8his
Plasmid#149269PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-8his
Plasmid#149270PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v3-8his
Plasmid#149271PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 3DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.2-8his
Plasmid#149663PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 2.2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 6021 - 6040 of 16015 results