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Plasmid#100798Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19GDepositorHas ServiceAAV1InsertTVA, EGFP, tet transactivator
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HA-hUBE1L
Plasmid#12438DepositorInsertUBE1L (UBA7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCasRx-ccdB
Plasmid#221003PurposeDestination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWP78
Plasmid#61263PurposeSmall IncP-based broad host range replicating vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EFS-Cas9-BlastR
Plasmid#115146PurposeSafe harbor site 231 knock-in vector with Cas9-BlastR expression cassetteDepositorInsertCas9-T2A-BlastR
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPT119
Plasmid#85526PurposeRepressilator without degradation tags, with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_ELAVL1
Plasmid#106104PurposeInducible gene expression of FlagHA ELAVL1DepositorInsertELAVL1 (ELAVL1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPIDNB
Plasmid#169901PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTSDepositorTypeEmpty backboneUseAivian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p45sheLL
Plasmid#37323DepositorInsertHPV45L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
p52sheLL
Plasmid#46950PurposeHPV52 L1 and L2 Bicistronic, too large to self-packageDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0728
Plasmid#143434PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIP (MLXIP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Mef2c-tPT2A-GFP
Plasmid#111771PurposeBicistronic construct: Co-express two genes by 2A peptidesDepositorInsertMef2c-tPT2A-GFP (Mef2c Mouse)
UseCloning intermediateTagsGFPExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-TetO-Pax7-IRES-EGFP
Plasmid#109372PurposeExpresses Dox-inducible Pax7 together with a GFP reporterDepositorInsertPax7
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT6
Plasmid#102325PurposeHuman SIRT6 for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-BirA-V5-neo
Plasmid#100548PurposeExpresses C-terminal V5-tagged BirA biotin ligaseDepositorInsertC-terminal V5-tagged BirA
UseTagsV5, HisExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Positron-ST
Plasmid#129254PurposeExpress soma-localized Positron in mammalian cellsDepositorInsertPositron-ST
UseTagsExpressionMammalianMutationD92N, E199VPromoterCAGAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver4.1
Plasmid#176544PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver4.1
Cas12f-GE ver4.1
UseTagsExpressionMammalianMutationPromoterU6 and chicken β-actinAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-hygro-mCherry-RAMP4
Plasmid#118391Purposestable lentiviral expression of cDNADepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLPT20
Plasmid#85460PurposeRepressilator with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsssrAExpressionBacterialMutationPromoterPLlac, PLtet, PR, and PRNAIAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SFFV-SMASH-CTERM-GFP
Plasmid#185766PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-OMP25
Plasmid#141150PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Rat)
UseTagsAcGFPExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RRM2
Plasmid#13796DepositorInsertRRM2 (RRM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
CKAR
Plasmid#14860DepositorInsertC kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsp27 wt FL
Plasmid#17444DepositorInsertsmall heat shock protein27 (HSPB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only