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Addgene
Showing: 6021 - 6040 of 16015 results
  1. pShuttle-CMV-F2A-T2A-Venus

    Plasmid
    #62621
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
    Depositor
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pET28a-mxLOX1

    Plasmid
    #104975
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pET28a-mxLOX2

    Plasmid
    #104976
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pBABE.puro/TO/Flag/mKO2

    Plasmid
    #110353
    Purpose
    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag
    Insert
    monomeric Kusabira Orange 2
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Availability
    Academic Institutions and Nonprofits only
  6. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. pSLIK 3xFLAG-NUP37 neo

    Plasmid
    #220361
    Purpose
    Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37
    Depositor
    Insert
    NUP37 (NUP37 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. LEMD3_BirA_Cterm

    Plasmid
    #221517
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. LEMD3_BirA_Nterm

    Plasmid
    #221518
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 6021 - 6040 of 16015 results