-
Plasmid#199137PurposeThis plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genomeDepositorInsert100 bp barcoding cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-PV_3C
Plasmid#203472PurposeExpresses PV 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertPV 3C protease (PVgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-HIV-2_PR
Plasmid#201935PurposeExpresses HIV-2 protease from a GAL-1x promoter with a URA3 markerDepositorInsertHIV-2 protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER4R
Plasmid#185884PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREP41xN_ccdB
Plasmid#79023PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xN_ccdB
Plasmid#79024PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM9,10
Plasmid#170280PurposeExpresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertUseTagsExpressionBacterialMutationPromoterTim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…Available sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETcon_SARS-CoV-2_RBD
Plasmid#166782PurposeYeast surface display vector, expressing the SARS-2 RBD, 201aa constructDepositorInsertSpike receptor binding domain (RBD) from SARS-CoV-2 (S )
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
mAID-BRD4 donor
Plasmid#140650PurposeBRD4 tagging with mAIDDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRD4-N CRISPR
Plasmid#140651PurposeBRD4 tagging CRISPRDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZ699
Plasmid#208117PurposePayload yeast assembly vector with TK-mActb on backboneDepositorTypeEmpty backboneUseSynthetic Biology; Copy number inducibleTagsExpressionBacterial, Mammalian, and Y…MutationPromoterHuman PGK1 for thymidine kinaseAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3T911A
Plasmid#120254PurposeExpression of ATP3-6 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
UseTagsExpressionYeastMutationT911 changed to A; Isoleucine 304 changed to Aspa…PromoterATP3Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-G418-YR
Plasmid#61767PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).DepositorInsertsnptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG4kwh
Plasmid#44722DepositorInsertspCMV-D2i promoter
htetR::NLS::EGFP
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only