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pMSCV-IRES-hCD2-CD14 (4R)

Plasmid

#133062

Purpose

Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (4R) (Cd14 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

CD14 allele defective for LPS binding (mutations …

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-PDL1

Plasmid

#159280

Purpose

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Human PDL1/CD274 (CD274 Human)

Use

AAV; S1 knockin donor vector

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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miniSOG-LaminB1-10

Plasmid

#57771

Purpose

Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LaminB1 (LMNB1 Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

Tet-On 3G

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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miniSOG-Golgi-7

Plasmid

#57768

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-IRES-GFP/CREB3L1

Plasmid

#214767

Purpose

Mammalian expression of human CREB3L1

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

CREB3L1 (CREB3L1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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miniSOG-ER-5

Plasmid

#57766

Purpose

Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

ER (CALR Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Venus-Bcl2-pEGFP-C1

Plasmid

#177417

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Aquifex aeolicus, Rat)

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Actin-7

Plasmid

#55085

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Vimentin-7

Plasmid

#57800

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Expression

Mutation

Promoter

lac

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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miniSOG-Alpha-V-Integrin-25

Plasmid

#57763

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-miniSOG-EB3-7

Plasmid

#55089

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLB2 5′ mouse CNS

Plasmid

#49348

Purpose

luciferase reporter with mouse Bmp2 promoter and 3' conserved noncoding sequence (CNS)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' CNS (conserved noncoding sequence) (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

-

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
miniSOG-EB3-7

Plasmid

#57765

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits