Addgene

We narrowed to 16,015 results for: myc tag

Showing: 5981 - 6000 of 16015 results

Results per page:

pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Use

Tags

His tag (6x)

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM304_YFP-IqgC_mRFP-Raf1(RBD)

Plasmid

#128698

Purpose

simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Inserts

iqgC (full length)
Raf1 kinase (aa 50-132)

Use

Dictyostelium discoideum expression vector

Tags

YFP and mRFP

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-AR-LBD

Plasmid

#171239

Purpose

Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-S650A

Plasmid

#171245

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Ala (AR-S650A)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-S650D

Plasmid

#171246

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Asp (AR-S650D)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-W741C-S81A

Plasmid

#171248

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-W741C-S81D

Plasmid

#171249

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PE2-His (IK1822)

Plasmid

#170103

Purpose

T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

Depositor

Keith Joung

Article

Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis

Use

CRISPR

Tags

6xHis tag

Expression

Bacterial

Mutation

Bacteria codon-optimized nSpCas9 (H840A) & M-…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

Plasmid

#164845

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Use

Tags

8his tag and HA leader sequence

Expression

Mammalian

Mutation

Codon-optimized for human cell expression. Has N5…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Use

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

Plasmid

#153441

Purpose

E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc. XDocB binding mode B knock-out mutant

Use

Tags

3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

Expression

Bacterial

Mutation

Arginine 140 and methionine 144 were mutated to a…

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-SARS-CoV-2-S-RBD-8his

Plasmid

#145145

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Use

Tags

8his tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

Plasmid

#111451

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

Plasmid

#75122

Purpose

Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
p2XStrep-ATIC

Plasmid

#125685

Purpose

expresses human ATIC protein with an N-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ATIC (ATIC Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp1

Plasmid

#156465

Purpose

Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3a_Ub1

Plasmid

#156467

Purpose

Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge