Addgene

Showing: 581 - 600 of 781 results

Results per page:

Sort results by:

pDule2 - Mj Acd A9

Plasmid

#197652

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Use

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 AUP1 292-410

Plasmid

#185331

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule- Mj Acd A9

Plasmid

#197651

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Use

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL-p21UTRm1&2

Plasmid

#20880

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

Plasmid

#107163

Purpose

Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

Depositor

Jose Carlos Cameselle

Article

Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

Insert

F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-M2-BAIAP2 S366A

Plasmid

#31657

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 S366A (BAIAP2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Serine 366 to Alanine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG/Foxa1 e2.4

Plasmid

#105511

Purpose

Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (sgRNA, e2.4)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-BAIAP2 S366A

Plasmid

#31676

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 S366A (BAIAP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed Serine 366 to Alanine

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1aL-WT_ABCA3-GFP

Plasmid

#188548

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1aL

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-GG-hUbC-EBPF2-gRNA-FAM13A

Plasmid

#185550

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

FAM13A gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2702-AGER-gRNA1

Plasmid

#216471

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2704-AGER-gRNA3

Plasmid

#216473

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2705-AGER-eGFP

Plasmid

#216474

Purpose

donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

eGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-XPACK-DNAJC5 (L115R)- HaloTag

Plasmid

#205712

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag and XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DEAD XPACK-DNAJC5 (L115R)

Plasmid

#205718

Purpose

Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Dead XPACK (GCINIKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge