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Addgene
Showing: 581 - 600 of 1572 results

  1. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #167529
    Purpose
    To increase the length of the linker between Venus and Noxa-4E proteins
    Depositor
    David Andrews
    Insert
    PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    C25E, L29E, F32E, L36E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    167529-plasmid-map-sequence-id-347649

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  3. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    209768-plasmid-map-sequence-id-417214

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  5. pUC19-link-mVenus-FLAG:Kan

    Plasmid
    #211830
    Purpose
    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    mVenus-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211830-plasmid-map-sequence-id-418060

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  11. Venus-BclXL-ActA-pEGFP-C1

    Plasmid
    #177410
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177410-plasmid-map-sequence-id-348171

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  12. Venus-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177415
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177415-plasmid-map-sequence-id-348201

    Enlarge

  13. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

    Enlarge

  14. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

    Enlarge

  15. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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  16. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  17. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

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  18. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  19. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

    Enlarge

Showing: 581 - 600 of 1572 results