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Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
UseTagsno tagsExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-GFE3
Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-11
Plasmid#183358PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-11 (Casp4 Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_07_01
Plasmid#165014PurposeExpression of HLA-DRB1*07:01 cDNADepositorInsertHLA-DRB1*07:01 (HLA-DRB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYU-2-Env
Plasmid#133997PurposeEnv gene from HIV-1 YU-2 in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV miRNA control plasmid
Plasmid#31508DepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCoofy44
Plasmid#55185PurposeInsect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Gateway-DSCP
Plasmid#71506PurposeflySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
YSEHPTFTSQY_epitope
Plasmid#165016PurposeExpression of CMV UL83 (363-373) fused to IL-2 signal sequenceDepositorInsertCMV UL83 (363-373)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRAC
Plasmid#164993PurposeExpression of gRNA targeting TCRalpha constant locusDepositorInsertgRNA against TRAC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-and1 in situ probe
Plasmid#52102Purposeactinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 polDepositorInsertactinodin 1 in situ probe (and1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4-Gateway-SCP1
Plasmid#71510PurposehumanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq validation vectorTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_1
Plasmid#164986PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_2
Plasmid#164987PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRBC
Plasmid#164994PurposeExpression of gRNA targeting TCRbeta constant locusDepositorInsertgRNA against TRBC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pES.HSV2.3a
Plasmid#69593PurposeTCR a chain construct for generation of gBT-I miceDepositorInsertTCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterH-2KbAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
VYAIFIFQL_epitope
Plasmid#165018PurposeExpression of CMV US20 (29-37) fused to IL-2 signal sequenceDepositorInsertCMV US20 (29-37)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only