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Addgene
Showing: 581 - 600 of 752 results

  1. pcDNA3-Mcm2-mCer

    Plasmid
    #211397
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211397-plasmid-map-sequence-id-420430

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  2. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211441-plasmid-map-sequence-id-419424

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  3. pcDNA3-C17orf53_1-241-mCer

    Plasmid
    #211443
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211443-plasmid-map-sequence-id-419423

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  11. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  20. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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Showing: 581 - 600 of 752 results