These results may also be relevant.
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pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-MKP-3 C293S
Plasmid#131525PurposeExpresses MKP-3 with C293S mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Cysteine 293 to SerinePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mMLKL
Plasmid#208784PurposeInducibly expresses mouse MLKL fused to light-activatable Cry2DepositorInsertmCherry mMLKL Cry2 (Mlkl Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-Itch/2793
Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-myc-Itch/2859
Plasmid#11427DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330.Pten.b
Plasmid#66588PurposesgRNA for Pten deletionDepositorInsertPten deletion (Pten Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330.Pten.a
Plasmid#66587PurposesgRNA for Pten deletionDepositorInsertPten deletion (Pten Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
UseTagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterU6 promoterAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIPi P2G_Thy1.1 PTEN
Plasmid#19749DepositorInsertoligo targeting PTEN (Pten Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag dPDZ-PTEN (1-400)
Plasmid#22232DepositorInsertPTEN (Pten Mouse)
UseTagsFlagExpressionBacterial and MammalianMutationPDZ binding domain deleted. Contains amino acids …PromoterAvailable sinceMarch 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-181a1-Dusp6
Plasmid#17849DepositorInsertDusp6-EGFP (Dusp6 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
p35S::IRE1a
Plasmid#135232Purposeconstitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)DepositorInsertIRE1a (IRE1A Mustard Weed)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Scara3-IRES-BFP
Plasmid#117265PurposeRetroviral overexpression of Scara3DepositorInsertScara3 (Scara3 Mouse)
UseRetroviralTagsExpressionMammalianMutationSee depositor comments below.PromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
