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Showing: 41 - 60 of 634 results

  1. pCPP6450

    Plasmid
    #128731
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752
    Insert
    HopAD1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    128731-plasmid-map-sequence-id-252571

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  3. pSG069

    Plasmid
    #214260
    Purpose
    pJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaI
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214260-plasmid-map-sequence-id-432474

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  4. pSG058

    Plasmid
    #214261
    Purpose
    PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214261-plasmid-map-sequence-id-432467

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  7. pCPP5233

    Plasmid
    #128715
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcM-hopM1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128715-plasmid-map-sequence-id-252574

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  8. pSG068

    Plasmid
    #214259
    Purpose
    pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    18 nucleotide 5' of the start codon (Shine-D…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214259-plasmid-map-sequence-id-432475

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  9. pSG059

    Plasmid
    #214262
    Purpose
    PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
    Use
    Tags
    6x His-tag and 6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214262-plasmid-map-sequence-id-432470

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  10. pfMDH_WT

    Plasmid
    #67721
    Purpose
    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pET20b_Pa-PirA

    Plasmid
    #128956
    Purpose
    expresses Pseudomonas aeruginosa PirA in E. coli
    Depositor
    James Naismith
    Article
    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24
    Insert
    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    Use
    Tags
    6xHis and pelB leader
    Expression
    Bacterial
    Mutation
    Signal peptide sequence has been removed
    Promoter
    T7
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128956-plasmid-map-sequence-id-254679

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  15. pSG071

    Plasmid
    #214257
    Purpose
    Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214257-plasmid-map-sequence-id-433134

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  16. pSG075

    Plasmid
    #214258
    Purpose
    Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214258-plasmid-map-sequence-id-432476

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  18. pACB109

    Plasmid
    #222208
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (K10N)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Lys10 to Asn
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222208-plasmid-map-sequence-id-437369

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  19. pACB110

    Plasmid
    #222209
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Gly258 to Aspartate
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222209-plasmid-map-sequence-id-437372

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  20. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcE-avrE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128703-plasmid-map-sequence-id-252488

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Showing: 41 - 60 of 634 results