Addgene

We narrowed to 1,538 results for: nif

Showing: 41 - 60 of 1538 results

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Use

Tags

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pME.A (pDPL6)

Plasmid

#24571

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pME.E (pDHg16)

Plasmid

#24573

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

pME.E (pDHg16)

Use

Tags

Expression

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pME.C (pDHf14)

Plasmid

#24572

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

Use

Tags

Expression

Mutation

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP

Plasmid

#74203

Purpose

Retroviral control vector containing GFP insert

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-HA3-STONIN1

Plasmid

#177580

Purpose

Expresses Stonin1 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

STONIN1

Use

Tags

3XHA and GFP

Expression

Mammalian

Mutation

V334A- please see depositor comments

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-STONIN2

Plasmid

#177885

Purpose

Espresses Stonin2 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

stonin2 (STON2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-eab2-[GTR]

Plasmid

#46144

Depositor

Wenbiao Chen

Article

Boniface et al Genesis. 2009 Jul;47(7):484-91. doi: 10.

Insert

EGFP/mCherry

Use

Cre/Lox; Tol2 zebrafish transgenic expression

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ4-(HA)3

Plasmid

#198180

Purpose

Expression of HA-tagged AP-4 μ4 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-4 μ4

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

Vps50 (Vps50 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-AP-4 σ4

Plasmid

#198328

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiblast-Paxillin-mTurqoise

Plasmid

#74206

Purpose

Lentiviral expression of Paxillin-mTurqoise

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

Paxillin

Use

Lentiviral

Tags

mTurqoise

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps53-V5-6His

Plasmid

#175097

Purpose

V5 tagged Vps53 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps53 (Vps53 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET16better_H10_HRV3C_chitinase

Plasmid

#175091

Purpose

Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

Depositor

Jay C Dunlap

Article

Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

Insert

chitinase

Use

Tags

10X His, HRV-3C

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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RUFY1-GFP

Plasmid

#197394

Purpose

Expresses RUFY1 in mammalian cells

Depositor

Juan Bonifacino

Article

Keren-Kaplan et al Nat Commun. 2022 Mar 21;13(1):1506. doi:

Insert

RUFY1 (RUFY1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMVf

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Rabaptin-5

Plasmid

#199173

Purpose

Expresses GFP-Rabaptin-5 fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

Rabaptin-5 (RABEP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains Tyr 149 Ser substitution; insert starts …

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

RLuc8

Use

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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