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pAAV-CAG-mTurquoise2

Plasmid

#99122

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mTurquoise2-CNA35

Plasmid

#61602

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mTurqouise and CNA35

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Rab7

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBABEpuro mTurquoise2 LC3B

Plasmid

#78518

Purpose

Fluorescent reporter for LC3B visualization

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

retroviral

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mKOkappa-2A-mTurquoise2

Plasmid

#98837

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKOkappa-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mKO2-2A-mTurquoise2

Plasmid

#98838

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKO2-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2-iLID

Plasmid

#176125

Purpose

Plasma membrane localized iLID

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-SNAPf-mTurquoise2

Plasmid

#197494

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn1-2xNLS-mTurquoise2

Plasmid

#118025

Purpose

An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

hSyn1

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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HT7-mTurquoise2-KDEL

Plasmid

#188571

Purpose

Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

Depositor

Pablo Rivera-Fuentes

Insert

HaloTag7

Use

Tags

KDEL and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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