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Plasmid#74260PurposeMammalian expression of C-terminal fusion of mClover3 to CX43DepositorInsertConnexin 43
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-humanCTCF-miniAID-mClover3
Plasmid#136883Purposeknockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCFDepositorInsertCTCF-knockin-homology arm-miniAID-mClover3
UseTagsminiAID-mClover3ExpressionMammalianMutationPromoterno promoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-V5-mClover3-pA (JDW 1318)
Plasmid#224530PurposeA Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyADepositorInsertV5-mClover3-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmClover3-hsLem2
Plasmid#179524PurposeExpress OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoterDepositorInsertLEMD2 (LEMD2 Human)
UseTagsmClover3-mAIDExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-bNup98-DN-NLS-mClover3
Plasmid#171495PurposeT7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNADepositorInsertNup98-DN (NUP98 B. tarus (bovine))
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-mClover3-CD4-bla
Plasmid#179441PurposeDonor vector to knock in mClover3 C-terminal to human CRY1 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9-T2A-mClover3
Plasmid#172293PurposeIn vitro transcription of Cas9-T2A-mClover3 synthetic constructDepositorInsertCas9-T2A-mClover3
UseCRISPRTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mClover3-CD4-bla
Plasmid#179448PurposeDonor vector to knock in mClover3 N-terminal to human PER2 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
C232-E29: attB2r-mClover3-attB3
Plasmid#162906PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteinsDepositorInsertmonomeric Clover3 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2zmiRFP-EF1-2xCMV-EF1-mClover3
Plasmid#196112Purposemammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3DepositorInsertzmiRFP-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3
Plasmid#196111Purposemammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3DepositorInsertzmiRFP2-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3
Plasmid#196109Purposemammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3DepositorInsertzTagRFP658-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3
Plasmid#196110Purposemammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3DepositorInsertzemiRFP2-EF1-2xCMV-EF1-mClover3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mClover3-stop
Plasmid#186389PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mClover3-RfA
Plasmid#186379PurposeDestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7G)-PuroR-F2A-mClover3
Plasmid#172291PurposeIn vitro transcription of PuroR-F2A-mClover3 synthetic constructDepositorInsertPuroR-F2A-mClover3
UseOtherTagsExpressionMutationPromoterT7 promoter G-initiatingAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138129PurposeExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmClover3-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2
Plasmid#105801PurposeConcomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: FLAG-3C; ORF2: mClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
C512-E22: attB5r-mClover3-nostop-attB1r
Plasmid#162944PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteinsDepositorInsertmonomeric Clover3 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only