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Plasmid#48952PurposeFor inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only
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pBEG R3-L4 #BGV194
Plasmid#48954PurposeFor inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUAL SRBI (GFP)
Plasmid#86979PurposeLentiviral expression construct encoding SR-B1 and GFP from separate promotersDepositorInsertSCARB1 (SCARB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFS and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R3) #BGV141
Plasmid#48958PurposeRetroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorTagsExpressionMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUAL CD81 (GFP)
Plasmid#86980PurposeLentiviral expression construct encoding CD81 and GFP from separate promotersDepositorInsertCD81 (CD81 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+3
Plasmid#121176PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDUAL CLDN (GFP)
Plasmid#86981PurposeLentiviral expression construct encoding Claudin-1 and GFP from separate promotersDepositorInsertCLDN1 (CLDN1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+4
Plasmid#121177PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-dsRed-L3 #BGV203
Plasmid#48973PurposeSupplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertdsRed
UseGateway cloning vectorTagsIRES (strong)ExpressionMutationPromoternoneAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iBlast-L3 #BGV204
Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iHygro-L3 #BGV206
Plasmid#48985PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserthygromycin phosphotransferase
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iCreT2ALuc-L3 #BGV208
Plasmid#48992PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertscre recombinase
Luciferase
UseLuciferase; Gateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterUnknownAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iPuro-L3 #BGV205
Plasmid#48987PurposeSupplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertpuromycin resistance
UseGateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eGFP-L3 #BGV201
Plasmid#48979PurposeSupplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteGFP
UseGateway cloning vectorTagsIRES (strong)ExpressionMutationPromoterNAAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only