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Showing: 41 - 60 of 259 results

  2. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  8. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107737
    Purpose
    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107737-plasmid-map-sequence-id-207281

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  10. pRVdG-4GCaMP6s

    Plasmid
    #52497
    Purpose
    Expresses GCaMP6s
    Depositor
    Ian Wickersham
    Insert
    GCaMP6s
    Use
    Rabies virus
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    52497-plasmid-map-sequence-id-81433

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  12. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107736
    Purpose
    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107736-plasmid-map-sequence-id-207278

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  13. spa-GCaMP6f

    Plasmid
    #67558
    Purpose
    photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Tags
    HIS-tag
    Expression
    Mammalian
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. spa-GCaMP6m

    Plasmid
    #67557
    Purpose
    photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6m
    Use
    Tags
    HIS-tag
    Expression
    Mammalian
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 259 results