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pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationR691APromoterEF1aAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p201B Cas9
Plasmid#59177PurposeCas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
BAR
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9 MDC123
Plasmid#59184Purpose2x35S promoting Glycing Max codon optimized Cas9 with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoter2X35SAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL010097 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Adeno Cas9
Plasmid#64072PurposeAdenoviral vector for the expression of the Cas9DepositorTypeEmpty backboneUseAdenoviralTagsFLAGExpressionMutationPromoterU6 and CBhAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorInsertCas9
UseCRISPR; Xenopus and zebrafish expressionTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMK33/Cas9
Plasmid#133781PurposeExpresses drosophila-optimized Cas9DepositorInsertDrosophila-optimized Cas9
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myh6-Cas9
Plasmid#109037PurposeCardiac-specific overexpression of Cas9DepositorInsertCas9
UseTags3XFlagExpressionMammalianMutationPromoteralpha MHCAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PE-Cas9
Plasmid#174851PurposeExpress PE-Cas9 in mammalian cellsDepositorInsertPE-Cas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9-GFP_sg_mTfeb
Plasmid#79006PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.DepositorInsertsgTfeb (Tfeb Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sniper-Cas9
Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only
